Products/Services Used | Details | Operation |
---|---|---|
Biochemicals> | … GSH and g-EC standards were acquired from Sigma and PC2 was purchased from GenScript Corporation (Piscataway, NJ). Peaks obtained for GSH, g-EC, PC2, PC3, and PC4 standards were used to identify the corresponding peaks of the samples … | Get A Quote |
Arsenic is one of the most hazardous pollutants found in aqueous environments and has been shown to be a carcinogen. Phytochelatins (PCs), which are cysteine-rich and thio-reactive peptides, have high binding affinities for various metals including arsenic. Previously, we demonstrated that genetically engineered Saccharomyces cerevisiae strains expressing phytochelatin synthase (AtPCS) produced PCs and accumulated arsenic. In an effort to further improve the overall accumulation of arsenic, cysteine desulfhydrase, an aminotransferase that converts cysteine into hydrogen sulfide under aerobic condition, was co-expressed in order to promote the formation of larger AsS complexes. Yeast cells producing ... More